Our equipment

High Performance Liquid Chromatography (HPLC) is a powerful instrument to purify a variety of materials, such as proteins, peptides, metabolites, etc. A typical HPLC system comprises a solvent delivery system, a sample injection valve, a high-pressure column, a detector, and a computer to control the system. HPLC uses high pressure to force solvent through closed columns containing microporous particles that give high-resolution separations. Typical separation is based on selective distribution of components in a mixture--between the liquid mobile phase and the stationary phase that is covalently bound to the inner wall of the column. Compounds that interact strongly with the stationary phase migrate slowly in the mobile phase. In contrast, compounds that are weakly retained by the stationary phase migrate quickly. In terms of different mobility of the components in the mixture, the sample components are separated into discrete bands that elute out at specific retention times. These separated bands can be analyzed both qualitatively and quantitatively using appropriate detectors.